Fig. 4. tsh expression induces ato. (A-C) tsh-GAL4; UAS-GFP L3 discs (tsh>GFP, red) stained with anti-Ato (green) and 22C10 (neural marker, blue). (A) In the eye disc, tsh>GFP expression extends up to the MF (arrowhead) and overlaps ato. Posterior to the MF, 22C10 marks differentiating photoreceptors. (B,C) tsh-GAL4; UAS-GFP is expressed in proximal domains in both leg (B) and wing (C) discs. (B) Leg disc showing Ato expression in a cluster of chordotonal organs (asterisk in B and E). (C) Wing disc showing a small cluster of Ato+ cells in the ventral anterior hinge region (asterisk). (D-F) In tsh-expressing clones (tsh+, red) some cells turn on Ato expression in the eye disc (D), leg (E) and wing (F) discs (arrows). The arrowheads in D indicate the ato expression at the endogenous MF.