Fig. 5. wwp-1 and alx-1 are required for LIN-12 degradation. All pictures are ventral views. In panels A-F, experiments were carried out with hermaphrodites that express LIN-12(+)::GFP. (A) The negative control lacZ(RNAi) does not affect LIN-12 downregulation in the 1° lineage. (B) lacZ(RNAi) does not prevent lateral signaling, as evidenced by the presence of AJM-1-positive granddaughters of P5.p and P7.p. (C) alx-1(RNAi) inhibits degradation, but not internalization (arrowheads). (D) alx-1(RNAi) does not prevent lateral signaling. (E) wwp-1(RNAi) inhibits degradation but not internalization, as LIN-12(+)::GFP accumulates in endocytic puncta (arrowheads). (F) wwp-1(RNAi) does not prevent lateral signaling. (G,H) For comparison, LIN-12(DTS)::GFP is not downregulated, and inhibits lateral signaling. (I) Quantification of downregulation and lateral signaling in RNAi experiments. Each set was performed in parallel three different times. Each time, 30 hermaphrodites at the Pn.px and Pn.pxx stages were scored for presence of GFP (gray bars) and 2° fate defects (black bars). Scale bars: 10 µm.