Fig. 1. CYC protein binds to DNA. (A) Specificity of CYC recombinant protein binding to class I and class II TCP consensus binging sites. Oligonucleotide probes contain a shared consensus binding site for class I GGNCCCAC (red) and class II GTGGNCCC (blue) to which CYC protein binds (arrow; lane 1). Bold underlined nucleotides indicate mutations introduced that affect both class I and II binding (lanes 2 and 3) or specifically only class II (lane 4) or class I (lane 5). (B) Consensus DNA-binding sequence for CYC protein obtained by a random binding site selection method. After five rounds of selection, a total of 31 binding sites of CYC were piled up using MEME (meme.sdsc.edu) and a consensus sequence generated. Nucleotides that were not occupied by a specific residue are indicated by N.