Fig. 2. Interaction of CYC recombinant protein with RAD promoter and intron. (A) RAD genomic region showing three potential CYC-binding sites in the promoter and intron. (B) PCR fragments of promoter and intron sequences with putative CYC-binding sites were amplified and used as probes in an EMSA with recombinant CYC protein. + or – indicates the presence or absence of CYC protein; asterisks indicate retardation of the mobility of CYC-DNA binding complex.