(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Shh inactivation in the peripheral retina is associated with increased cell cycle exit and depletion of the RPC pool. (A-L) In situ hybridization on wild-type and {alpha}-Cre;Shh–/c retinas at E14 and P0. (A-D) Gli and (E-H) cyclin D1 expression are downregulated in the peripheral retina of mutant mice at both ages (between arrowheads in B,D,F,H). (I-K) In situ hybridization for Rax expression to mark the neuroblast layer, which by P0 is reduced in thickness in the peripheral retina of the mutant mice (between the arrowheads in L). (M) The number of S-phase cells is reduced in the peripheral retina of the mutant mice at E14. (N) The number of birthdated cells is increased in the peripheral retinas of mutant mice. RPC were labelled at E13 with BrdU and the number of heavily labelled nuclei was counted in the central versus peripheral retina at P0. *P<0.005. Scale bar: 100 µm.