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Fig. 2. Localization of dnpc1a RNA in embryos and larvae. (A-E) Anterior towards the left. (A) Ubiquitous expression of dnpc1a in a cellular blastoderm-stage embryo. (B) Higher level of expression can be seen in gastrulation furrow (arrow) of stage 7 embryo. (C) After germband extension, higher level expression is in the hindgut (arrow). (D) Aminoserosa (arrow) staining can be seen in a stage 14 embryo. (E) Putative prothoratic gland cells (arrow) in stage 16 embryos. (F) High level expression of dnpc1a mRNA in the ring gland of wandering third-instar larvae. (G) dnpc1a mRNA is also present in the garland cells (arrow). (H) In situ hybridization with dnpc1a probe detects no signals in dnpc1a1 mutant embryo.