Fig. 6. Sterol accumulation in dnpc1a mutants. (A,B) Filipin-stained wing imaginal discs from wild type (A) and dnpc1a mutant (B) third-instar larvae. Insets show magnified views; sterol is mainly located at cell-cell boundaries in wild type, while it accumulates in a punctate pattern in dnpc1a mutants. (C,D) Filipin-stained brains from wild-type (C) or dnpc1a mutant (D) third-instar larvae. There is no punctate accumulation present in wild type (C), but some accumulation can be seen in dnpc1a mutants (arrows in D). Insets show magnified views. (E-H) Third-instar ring glands (outlined) in different backgrounds. (E) Wild-type ring gland filipin staining. (F) Filipin staining of ring gland in ecdysone-rescued dnpc1a mutants. Punctate sterol accumulation can be seen in both ring gland and brain lobes. (G) The 2-286 driver specifically induces the expression of dnpc1a-YFP in ring gland but not brain lobes. In order to see the brain lobes, the signal was boosted. (H) dnpc1a mutant rescued by 2-286 driving dnpc1a-YFP. The ring gland shows a wild-type filipin staining pattern, while the brain lobes still have punctate sterol accumulation.