Fig. 1. ATBF1 expression is associated with neuronal differentiation in E14.5 rats. (A) BrdU (green) and ATBF1 (red). ATBF1 is expressed in the ganglionic eminence (GE), medial tegmentum (Tg), isthmus (I), pons (P), colliculus (C), spinal cord (S), tongue (T) and elsewhere. (B) mRNA expression in embryonic and postnatal rat brains evaluated by real-time RT-PCR. (C1-F2) Magnification of the GE and further magnification of the framed area. (C1) BrdU labeling in the VZ and SVZ. ATBF1 is present in the differentiating field (DF) but not in the white matter (W). (C2) Higher magnification of the boxed area in C1. (D1) Nestin is present in the VZ and SVZ extending a fibrous network from the SVZ into the DF, but is segregated from ATBF1-positive cells. (D2) Higher magnification of the boxed area in D1. (E1) ß-tubulin III is present in the DF with ATBF1. (E2) Higher magnification of the boxed area in E1. (F1) MAP2 is present in the DF with ATBF1. (F2) Higher magnification of the boxed area in F1.