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Fig. 2. Overexpression study of ATBF1 cDNA in primary cultured neuroepithelial cells. (A1-5) Immunohistochemistry for nestin (green) overlaps with the HA-tag (red) after transfection of an HA-tagged mock expression vector. (B1-5) Nestin (green) expression is suppressed by HA-tagged ATBF1 (red) expression. (C1-5) ß-Tubulin (green) does not overlap with an HA-tagged (red) mock expression vector. (D1-5) ß-tubulin (green) expression overlaps with HA-tagged ATBF1 (red) expression. (E) A nestin-specific enhancer element is suppressed by ATBF1. (F) The Neurod1 promoter is activated by ATBF1. *P<0.01.