Development -- Sen et al. 132 (23): 5147 Figure IG2

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Fig. 2. Comparison in eye size and proliferation between RCAS-DNhRAR ${alpha}$ infected and uninfected eyes. (A) Two eyes from one E7 embryo, injected in the right eye with RCAS-DNhRAR ${alpha}$ at HH stage 17. Uninjected control eye on the left and the injected eye on the right. White arrowhead shows ventral fissure region of injected eye. (B,D) Dissociated cells from retina injected with RCAS-DNhRAR ${alpha}$ or (D) with RCAS-GFP, labeled with [³H] thymidine (dark grains) and 3c2 anti viral-gag antibody (red) and DAPI (blue). (C) Dissociated cell counts from 4 hour [³H] thymidine-labeled E7 retinae that were injected with RCAS-GFP control virus or RCAS-DNhRAR ${alpha}$ . The percentage of [³H] thymidine-labeled cells were counted among the infected, 3c2+ population (green bar and dark blue bars) as well as the uninfected, 3c2-population (red bars and light blue bars). The data for RCAS-DNhRAR ${alpha}$ are from nine infected retinae and the data for RCAS-GFP are from six infected retinae.