Fig. 2. Defective DTC migration in the src-1(cj293) mutant. The position of DTC was monitored by analyzing the expression of lag-2::gfp in wild-type (A,C,E,G) and src-1(cj293) mutant (B,D,F,H) worms. The DTCs migrate in opposite directions along the ventral body wall muscle during the L2 stage in both the wild-type and mutant (A,B). However, the DTCs fail to turn dorsally in the src-1(cj293) mutant (D) during the L3 stage and continue their centrifugal migration in the anterior and posterior directions (F). By contrast, in the wild-type worm at the adult stage, the DTCs migrate centripetally along the dorsal muscle bands back toward the midbody (E). In the adult src-1(cj293) mutant, the extended gonads are randomly bent and accordionated (H). The arrowheads indicate the DTCs. Arrows show the migration pattern of the DTCs. Scale bar: 100 µm.