Fig. 5. DTC expression of SRC-1 in the wild-type DTCs, and limited tyrosine phosphorylation in the DTCs of the src-1(cj293) mutant. (A.B) The expression of SRC-1 in wild-type DTCs was determined by detecting the expression of a reporter gene expressed under the src-1 promoter (Psrc-1::gfp). The DIC image is shown in B. (C,D) The dissected gonads of a wild-type worm (C) and the src-1(cj293) mutant (D) were stained with the anti-phosphotyrosine antibody 4G10. The arrowheads indicate the DTCs. Scale bar: 40 µm.