Fig. 6. (A,B) The expression of unc-5 is not affected by the src-1(cj293) mutation. The expression of unc-5::lacZ in wild-type worms (A) and the src-1(cj293) mutant (B) at around L3 stage was detected by staining for ß-galactosidase activity. The arrowheads indicate the DTCs. Scale bar: 100 µm. (C,D) The precocious turning of DTCs induced by the precocious expression of UNC-5 is not observed in the src-1(cj293) homozygote background. The expression vector for UNC-5 under the emb-9 promoter (emb-9::unc-5) was injected into the src-1/hT2 worm, and the gonad morphology of the resulting heterozygote (src-1/+; C) and homozygote (src-1/src-1; D) was analyzed. The arrows show the migration pattern of the DTCs. Scale bar: 40 µm.