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Fig. 8. Loss of Gli3 partially rescues the Col2a1-Ihh phenotype. Serial sections of E16.5 Gli3+/– (A,E,I,M,Q; control), Gli3–/– (B,F,J,N,R), Col2a1-Ihh;Gli3+/– (C,G,K,O,S) and Col2a1-Ihh;Gli3–/– (D,H,L,P,T) limbs of the same litter were hybridized with riboprobes as indicated. E-H are higher magnifications of the distal radii (boxed) in A-D. (E-H) The size of the zone of proliferating chondrocytes (red arrow) is reduced in Gli3–/– mice and strongly increased in Col2a1-Ihh;Gli3+/– mice. Compared to Col2a1-Ihh;Gli3+/– mice, the zone of proliferating chondrocytes is decreased in double transgenic Col2a1-Ihh;Gli3–/– mice. (I-L) The size of the domain of distal chondrocytes, demarcated by the Fgfr1 expression domain (green arrow), is slightly reduced in Col2a1-Ihh;Gli3+/– and strongly reduced in Gli3–/– and Col2a1-Ihh;Gli3–/– mice. (M-P) The Fgfr3 expression domain is expanded in Col2a1-Ihh;Gli3+/– limbs, whereas in double transgenic Col2a1-Ihh;Gli3–/– mice the Fgfr3 expression domain is of similar size to that in wild-type and Gli3–/– limbs. (Q-T) The upregulated level of PTHrP expression in Col2a1-Ihh;Gli3+/– mice seems to be downregulated in Col2a1-Ihh;Gli3–/– mice to similar levels as in control or Gli3–/– limbs.