Fig. 5. The QA motif is preferentially required for repression of postnotal tissue.
Postnotal genotypic series for T3 laterotergite transformation towards T2
thorax as assessed by number of ectopic bristles (blue arrows): (A)
Ubx+/Ubx+ > (B)
Ubx/Ubx+ > (C)
Ubx
QA/Ubx
QA
> (D)
Ubx
QA/Ubx.
The T3 laterotergite (green line) is normally a thin band of tissue separating
T2 and A1 but is expanded in mutants lacking the QA motif (surrounded by green
lines marking border). The junction of the haltere and the laterotergite is
marked with a green `T3' at the edge of the line. Anterior is upwards, lateral
is leftwards and the dorsal midline is rightwards. (A)
Ubx+/Ubx+ T3 half-laterotergites never had
bristles, while
Ubx
QA/Ubx (D)
always had bristles (up to a dozen). In the Oregon-R background
(shown above),
Ubx
QA/Ubx
QA
T3 half-laterotergites (C) had 0.972±0.592 bristles, whereas
Ubx/Ubx+ T3 half-laterotergites (B) had
0.017±0.091 bristles (P<1020;
n=90 and 60, respectively). In the WI129 background (not
shown),
Ubx
QA/Ubx
QA
T3 half-laterotergites had 0.167±0.290 bristles, whereas
Ubx/Ubx+ T3 half-laterotergites had
0.000±0.000 bristles (P<105;
n=90 and 60, respectively).