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Fig. 5. The N-terminal region of Ubx is required for ectopic activation of dpp in visceral mesoderm. (A-E) dpp transcripts in the visceral mesoderm of stage 13 embryos. (A) In wild-type embryos, dpp transcripts are detected in parasegments 4 and 7 (arrowheads). (B) Ectopic wild-type Ubx activates dpp transcripts anterior to and posterior to parasegment 7 (arrows). (C) Ectopic Ubx{Delta}2-19 protein barely activates ectopic dpp in parasegments 5 and 6, and represses endogenous dpp in parasegments 4 and 7 (arrowheads). (D) Ectopic Ubx{Delta}20-61 protein activates dpp transcripts anterior to, but not posterior to parasegment 7. (E) Ectopic Ubx{Delta}YPWM protein activates dpp transcripts in a pattern and amount indistinguishable to wild-type Ubx. (F) Quantitation of the ectopic Ubx protein levels and dpp transcripts in parasegments 5 and 6. Error bars: 95% confidence intervals.