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Fig. 2. Development of the areas of clearance. (A,B) CM1 labeling of stage 15 bicoid (A) and oskar (B) mutants. Open arrows indicate areas of clearance. (C-F) Confocal images of lateral views of stage 14 (C), stage 15 (D), stage 16 (E) and stage 17 (F) bicoid mutants stained with anti-Discs large (Dlg) antibody to visualize cell outline. Clearance of tissue is initially detectable in two distinct zones (see arrows in C and D), but becomes broader over time, fuses (E) and enlarges (F). Similar results were obtained for oskar (not shown). (G,H) DAPI staining of wild-type (G) and bicoid (H) mutants to visualize chromosomal DNA. The areas of clearance (arrow in H) do not contain DNA.