Fig. 1. pelo is required for controlling ovarian GSC self-renewal. All germaria or ovarioles in this and subsequent figures represent one confocal section and are shown with anterior towards the left. (A) A schematic diagram of the germarium. Red circles indicate spectrosomes (i.e. GSCs and cystoblasts) and branched red structures indicate fusomes (i.e. germ cell cysts). Ovarioles from pelo¹ homozygous mutant (C-E) and heterozygous control (B) flies labeled for Vasa (red, germ cells) and for Hts (green, spectrosomes and fusomes). (B-E) Germaria contain two GSCs (B) (indicated by arrowheads), two GSCs (C), one GSC (D) and no GSCs (E). (F) A pelo¹ mutant germarium labeled by TUNEL (red), showing that the GSC (arrowhead) was not apoptotic but there are some dying follicle cells (red) in the posterior. Scale bars: 10 µm.