Fig. 2. pelo functions in the cytoplasm of GSCs to control their self-renewal. The tips of the ovarioles (A-D) are labeled for lacZ (green) and Hts (red); the marked GSCs (broken lines) are identified by loss of lacZ expression and presence of a spectrosome on their anterior side. (A,B) Germaria showing the presence of 3-day-old (A) and 24-day-old (B) marked wild-type GSCs. (C) A germarium carrying a marked 3-day-old pelo¹ homozygous GSC. (D) A germarium showing loss of a marked pelo¹ mutant GSC evidenced by the presence of a marked germline cyst (arrowhead) in an egg chamber 17 days ACI. (E) Quantitative analyses of pelo¹ GSC loss and phenotypic rescues by different pelo transgenes, which carry wild-type or mutant (nls^*) epitope-tagged pelo. The x-axis shows different genotypes, while the y-axis indicates the average GSC number with a standard error. The blue bars indicate GSC numbers of the 2-day-old germaria of different genotypes, while the red bars represent GSC numbers of 7-day-old germaria. For each genotype at either time point, 40-130 germaria were examined. (F) A germarium with two GSCs (arrowheads) labeled for Hts (green) and DAPI (blue, nuclei) showing that germline expression of pelo is sufficient to rescue GSC loss in pelo¹ mutants. (G,H) Mutant germaria with one (G) or no (H) GSC labeled for hh-lacZ (red) and Hts (green), showing the normal number of cap cells. Scale bars: 10 µm.