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Fig. 3. Pelo is primarily localized in the cytoplasm of germ cells. (A-D) A nos-gal4VP16; UAS-FLAG-Pelo; Sec61a-GFP germarium labeled for FLAG (red, A) and GFP (green, B). The merged image (C) shows FLAG-tagged Pelo is localized primarily in the cytoplasm but is not particularly rich in ERs (green). (D) The enlarged area of a GSC marked in C. Scale bars: 10 µm.