Fig. 5. A mutation in pelo can allow bam mutant cystoblast-like cells to differentiate into cystocytes. Germaria are labeled for Hts (red, A,B) or Orb (red, C,D) and DAPI (nuclei, blue). (A) A germaria homozygous for bam⁸⁶ and heterozygous for pelo¹, showing undifferentiated single germ cells as evidenced by spectrosomes. (B) A germarium double homozygous for pelo¹ and bam⁸⁶ showing the formation of cystocytes, as evidenced by branched fusomes (arrowhead). In addition, many persistent spectrosomes (arrow) are abnormally enlarged. (C) A wild-type germarium showing preferential accumulation of Orb in the newly formed oocyte (arrowhead). (D) A double pelo and bam homozygous germarium showing no oocyte formation, which is suggested by no preferential Orb accumulation in germ cells. Scale bars: 10 µm.