Fig. 1. Phenotypes and distribution of GFP fluorescence in CPCp::GFP (A-C) and CPCp::CPC:GFP (D-F) transgenic plants. (A,D) Confocal laser scanning microscopy images of GFP fluorescence (green) and propidium iodide (PI) fluorescence (red) in the root epidermis of 5-day-old seedlings. (A) GFP is restricted to hairless cell files and is absent from the file of root hair cells (asterisk). (D) CPC:GFP has moved from hairless cell files to a hair cell file (asterisk; note CPC:GFP accumulation in the nuclei). The inset in D shows that CPC:GFP RNA was localized in hairless cells, as detected by in situ hybridization with an antisense GFP probe. (B,E) Distribution of root hairs in the root epidermis of 5-day-old seedlings. CPCp::CPC:GFP transgenic plants grow ectopic root hairs in positions normally occupied by hairless cells. (C,F) Phenotype of 8-day-old seedlings. No trichome formation was observed in CPCp::CPC:GFP transgenic plants (F). Scale bars: 50 µm in A; 200 µm in B; 1 mm in C.