Fig. 3. Western blot of whole-cell extracts and semi-quantitative RT-PCR analysis in Col-0 wild type (Wt), transgenic (CPCp::CPC:GFP, CPCp::HA:CPC cpc-1 and 35S::CPC) and cpc-2 seedlings. (A) CPC or CPC:GFP fusion proteins were detected with anti-CPC antibody, as indicated by the arrowheads. Molecular weights are shown on the right. Equal loading was confirmed by Ponceau staining of the blotted membrane. (B) Semi-quantitative RT-PCR analysis. One microgram of total RNA was used for each reverse transcription reaction. PCR: 26 cycles for CPC, CPC:GFP or HA:CPC; 22 cycles for the EF1 control.