Fig. 4. Localization of truncated CPC in cells of the root epidermis. (A) Schematic representation of CPC, consisting of three non-overlapping domains (N, N-terminal domain; Myb, Myb domain; C, C-terminal domain), and the four truncated forms of the CPC:GFP fusion protein. At the bottom, the construct consisting of the CPC promoter, a truncated CPC and 2xrsGFP is shown. (B-E) CLSM images showing GFP fluorescence (green) and PI fluorescence (red) in the root epidermis of 5-day-old seedlings. (B,D) NMG moved from hairless cells to hair cells (hair cell file is highlighted by an asterisk), whereas NG did not. (C,E) Reduced movement of MG and MCG was indicated by the patchy occurrence of GFP fluorescence in hair cells. NMG, MG and MCG accumulated in the nuclei (B,C,E), while NG was dispersed throughout the hairless cells (D). Scale bar: 50 µm.