Fig. 9. Inter- and intracellular movement of CPC fused to tandem GFPmers. (A-D) CLSM images of GFP fluorescence (green) and propidium iodide (PI) fluorescence (red) in the root epidermis of 5-day-old seedlings. The CPC promoter-driven CPC was fused to 2xGFP (A), 3xGFP (B), 4xGFP (C) and 5xGFP (D). GFP fluorescence was observed in epidermal cell nuclei with 2xGFP or 3xGFP fusions (A,B), but patchy GFP fluorescence in hair cells was observed in CPC:4xGFP and CPC:5xGFP lines (C,D). CPC fused to greater than 3xGFP reduced the nuclear-specific localization (C,D).