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Fig. 1. Fgfr1-IIIc and Fgfr2-IIIb isoforms are expressed by migrating PGCs. (A) cDNA prepared from FACS-sorted E10.5 PGCs and somatic cells was subjected to PT-PCR. The purity of the preparations was confirmed by expression of the PGC marker gene stella, and the somatic marker genes steel and Twist1. Lane 1, E10.5 PGC cDNA (PGC); Lane 2, E10.5 somatic cell cDNA (SC); Lane 3, E10.5 whole embryo cDNA (WE); Lane 4, RT(-) E10.5 whole embryo cDNA [WE(RT-)]. (B) The expression patterns of all FGFR isoforms were assayed by RT-PCR. Fgfr1-IIIc and Fgfr2-IIIb were found to be expressed by E10.5 PGCs (lane 1; lanes as in A). PGC-derived PCR products were sequenced and verified that these bands truly represented the expression of each FGFR. The illustration shows the potential FGF ligands that could activate each FGFR.