Fig. 3. Pixie is cytoplasmically localized and expressed uniformly in wing discs throughout development. Confocal images of wing discs immunostained with antiserum against Pixie. z sections are shown with x- and y-axis sections above and on the right respectively. The red line indicates the position of the y-section and the green line indicates the position of x-section. The blue line in the x- and y-sections depicts the position of the z-section. (A) Early third instar wing disc. (B) Wandering third instar disc. Additional panels above and on the right show in addition, propidium iodide staining (red), indicating the presence of nuclei in regions of the disc where Pixie staining is not detectable. Scale bar: 50 µm.