Fig. 6. Spatial and temporal dynamics of the effect of pix^L17 and eIF4A¹⁰⁰⁶ on wing disc clonal growth. (A) A schematic showing the different clone induction windows used. Larval stage and developmental time in hours AEL is shown below. Black bars above indicate start and end of each clone induction window; duration is shown in hours (h) on right of each bar. Windows representing the `fast' phase end at mid-third instar (pink box); those representing the `slow' phase end at larval wandering and are within the blue box. The 60-hour window spans both phases. The boxes overlap; however, there is a time lag between the time of clone induction and clone generation, and MDT analysis (D) reveals that the actual overlap in the life of the clones is likely to be minimal (see Materials and methods). (B,C) Images of pix^L17/GFP heterozygous discs with clones at mid-third instar (B) and a wandering third instar (C). The wing pouch, recognized as the unfolded epithelium at the centre of the disc, is marked by a white line; a blue line marks the DV boundary. The region regarded as hinge in our experiments surrounds the pouch and is within the red line. It includes regions that contribute to the ventral thorax, and, in early discs, the distal notum. (D-G) Bar charts in grey show the wild-type twin (GFP^+/+) MDTs. Black and blue bar charts show mutant/twin clone size as average (black bars) and median (dark blue bars) with unfilled bars on right of each chart depicting a ratio of 1, which would indicate no effect on growth, for comparison. When clones are frequently absent, the median clone/twin size is 0, or much smaller than the average clone/twin size. As shown in A, pink and blue boxes mark clones examined at mid third instar or at wandering, respectively; the region examined (pouch or hinge) and genotype of clones (pix^L17 and eIF4A¹⁰⁰⁶) is indicated. x-axis shows clone induction window lengths (h), the median number of divisions within the twins (red) and the number of twins analyzed (n). Fig. S4 in the supplementary material shows raw clone and twin cell number data for the above experiments.