Fig. 7. A Minute background has different effects on the growth of
pixL17 clones during fast and slow growth. (A,B)
Schematics showing the stages of development and clone induction windows used.
Blue boxes represent discs examined at larval wandering and pink boxes discs
examined at approximately mid-third instar. (C-L) Bar charts depicting clone
size distributions of pixL17, Minute+ clones
(C-J) and wild-type clones (K-L) as control. When
Minute+/+ (Minute+) clones are
generated in a Minute+/- (Minute) background, the
Minute-/- twins survive poorly and cannot be used to
assess background tissue growth. Thus, the size distribution of clones in a
Minute background (right) is compared with the size of
pixL17 or wild-type clones generated in a normal,
Minute+ background (left), using comparable clone
induction window lengths and stages of development.
Minute-/- twins survive better during the fast phase and
are shown as red bars in D. In the other experiments, these twins are rarely
found. For example, among the clones depicted in H, four in the hinge were
accompanied by twins. They are even more rare in the experiments depicted in F
and L and are not seen in J. Twin clones in the Minute+
background are not depicted because this data is shown in
Fig. 6. x-axes show
size categories of clone cell number and y-axes show number of clones
in each category. When sufficient numbers of clones are present, MDT is
indicated. Difference in clone growth between pouch and hinge in D,F and L is
significant, P<0.005 in D and F; P<0.000001 in L.