Fig. 5. lsy-2 is required for proper expression of feedback loop regulators. (A-C) No exp., no expression in ASEL and ASER; L>R, greater expression in ASEL versus ASER; L>0, expression in ASEL only; L=R, equal expression in ASEL and ASER. (A) Expression of lsy-6, assayed with the lsy-6^prom::gfp transgene otIs160, is lost in the ASEL neuron of lsy-2(ot64) null mutant animals. (B) ASER-specific expression of cog-1, assayed with the cog-1::gfp transgene syIs63, is de-repressed in ASEL in lsy-2(ot64) null mutant animals. (C) mir-273 mediated downregulation of the die-1 3'UTR in ASER, assayed with the ceh-36^prom::gfp::die-1^3'UTR transgene otEx1759, is disrupted in lsy-2(ot64) null mutant animals. (D) Placement of lsy-2 relative to the previously described bistable feedback loop in ASEL (left) and ASER (right). For a more detailed explanation of the regulatory interactions, see Johnston et al. (Johnston et al., 2005).