Fig. 1. Wingless is degraded in a lysosomal compartment. (A) HRP-wingless (dark stain) is detected in recognizable multi-vesicular bodies and lysosomes in wing imaginal discs. HRP-wingless was driven by dpp-Gal4, which is expressed in a broad stripe that lies perpendicular to the Wingless expression domain. This micrograph was taken outside the domain of dpp expression (in receiving cells). (B,C) Wingless protein (shown in C) accumulates in deep-orange (VPS18) mutant cells, which are recognized by the absence of ß-galactosidase (shown in B). (D) Accumulation of Wingless protein in cells that express shi[ts] under the control of the dpp-Gal4 driver, upon incubation for 3 hours at 32°C. (E,F). Imaginal discs expressing shi[ts] under the control of apterous-Gal4 (in the dorsal compartment; top half) fixed after a 5-hour incubation at 32°C. distalless expression is reduced in E and engrailed expression is reduced in F. (G,H) Increased stabilization of Armadillo upon reduction of dynamin-mediated endocytosis. Both panels show a disc overexpressing armadillo under the control of engrailed-Gal4 (in the posterior compartment). Both discs were kept at 32°C for 3 hours prior to fixation and staining with anti-Armadillo. (G) A shi[ts] heterozygote. (H) A shi[ts] hemizygote. Armadillo staining is increased in H.