Fig. 6. Loss of Sqd in the germline does not affect posterior follicle cell specification, and loss of Sqd in somatic cells does not affect their polarity. (A) Expression of the posterior follicle cell marker pnt^rm254 (blue) is normal in sqd^j4B4 germline clones, while kin-ß-gal is mislocalized (arrow). (B) The centripetal follicle cell marker BB127 (blue) is expressed normally at the anterior of sqd^j4B4 germline clones (arrow). (C) Baz protein (red) localizes to the apical membrane of follicle cells. The absence of nuclear GFP (green) marks sqd^j4B4 mutant clones. Note that this egg chamber is also mutant for sqd in the germline. Baz localization is normal in sqd^j4B4 follicle cell clones. (D) The wing blade trichome bristles display normal planar cell polarity in sqd^j4B4/Df(3R)urd escaper flies.