Fig. 3. hGFAP-cre is expressed in glial progenitor cells during postnatal development. (A) Sections from the thalamus of P8 control (a,c,e) and mutant (CKO) brains (b,d,f) were subjected to double-labeling immunofluorescence with anti-Cre (red) and anti-GFAP (green) (a-d), and anti-Cre (red) and anti-BLBP (green) (e,f). (B) Sections from the cerebellar white matter of P8 control (a,c,e) and mutant brains (b,d,f) were subjected to double-labeling immunofluorescence with anti-Cre/anti-GFAP (a-d) and anti-Cre/anti-BLBP (e,f). The mutant cerebellar white matter has significantly more GFAP-positive cells than control (marked by broken lines in a-d). Most of the Cre-positive/GFAP-negative glial progenitor cells are distributed in the periphery of the cerebellar white matter (outside the broken lines). Arrows indicate glial progenitor cells expressing Cre and BLBP, but not GFAP. Genotypes: control mice, Nf1^flox/+;hGFAP-cre+; mutant mice, Nf1^flox/-;hGFAP-cre+ or Nf1^flox/flox;hGFAP-cre+. Scale bars: 100 µm.