Fig. 5. A subset of Nf1^-/- astrocytes in the adult brain express nestin. Sections from adult control (A) and mutant (B) dentate gyrus (DG), as well as control (C) and mutant (D) cerebral cortex were subjected to double-labeling fluorescence with anti-nestin (red) and anti-GFAP (green). LV, lateral ventricle. Reactive astrocytes expressing both GFAP and nestin were observed only in the mutant brain (arrows in B,D). Immunohistochemical analysis with anti-P-erk of the control (E) and mutant (F) dentate gyrus. A population of nestin-positive reactive astrocytes (arrows in H, see inset for high magnification) was identified only in a subset of aged mutant brains, but not in control or mutant mice at young age (G). Scale bars: 100 µm.