Fig. 7. Nf1^hGFAPKO optic nerves are hyperplastic. Sections from control (A,C) and mutant (B,D) distal optic nerves and chiasms (asterisk) were stained with Hematoxylin and Eosin. Arrows in A,B indicate the enlarged area of the nerves shown in C,D, respectively. Adjacent sections from control (E,G) and mutant (F,H) optic nerves were stained with anti-nestin (red) and anti-GFAP (green). Mutant optic nerves are enlarged (B), hyperplastic (D) and disorganized (F,H). Sections from control (I) and mutant (J) proximal optic nerves were stained with Hematoxylin and Eosin. The most pronounced hypercellularity was observed in the proximal optic nerves (J). Scale bars: 100 µm.