Fig. 8. Nf1^hGFAPKO mice develop optic pathway gliomas. Two independent optic gliomas (B,C) are shown with Hematoxylin and Eosin staining and compared with the control (A). Adjacent sections from control (D) and mutant nerves with gliomas (E,F) were stained with anti-nestin (red) and anti-GFAP (green). Unlike the well-organized normal astrocytes that express both nestin and GFAP in control (D), most of the cells in optic gliomas express only GFAP (E,F, arrowheads). Scale bars: 100 µm.