Fig. 1. Immunohistochemical characterization of the MSCs. Phenotypical characterization of MSCs in the lower permanent portion of the hair follicle (top) and MCs in the hair matrix region (bottom) in stage 8 guard hairs. Skin sections were stained with antibodies against Dct, Tyr, Si, Tyrp1, Kit, Pax3, Mitf, Sox10, Lef1 or Mki67 (green), and with anti-ß-gal (red). Nuclear staining (blue) was with TO-PRO3. Arrowheads indicate MSCs in the lower permanent portion of the hair follicle. Scale bars: 10 µm.