Fig. 1. Bmp2 expression pattern during cardiogenesis. Whole-mount in-situ hybridization with Bmp2 exon3 probe shows Bmp2 expression pattern (A-I). At 7.5 dpc, Bmp2 expression can be visualized bilaterally in the primitive heart tube (A, indicated by arrows). Side view (B) and frontal view (C), indicating that at 8.5 dpc Bmp2 is expressed in the sinus venosus. At 9.5 dpc, both lateral view (D) and frontal view (E) showed that Bmp2 is highly expressed in the myocardium of the AV canal, as well as lower level of expression in the outflow tract (indicated by arrows). (F,G) Side view and frontal view, showing that at 10.5 dpc Bmp2 expression is maintained in the myocardium of the AV cushion region and in the outflow tract (arrows). At 11.5 and 12.5 dpc, Bmp2 expression decreased significantly, as denoted by arrows (H,I). A, atrium; LA, left atrium; LV, left ventricle; OFT, outflow tract; RA, right atrium; RV, right ventricle; V, ventricle.