Fig. 4. Western analysis of Vls. Ponceau Red staining serves as a loading control
and is shown below the corresponding blot. (A) Anti-Vls antibodies detect a
protein of 
42 kDa in wild-type ovaries and in ovaries expressing one copy
of the CG10728+/vls+ rescue construct.
This antigen is not detected in vlsnull mutants, nor in
vlsEMS hemizygotes and trans-heterozygotes. Genotypes:
vlsnull - Df(2L)be408 / Df(2L)pr2b,
P[w+ barr+];
vlsnull vls+ - Df(2Lbe408,
P[w+
CG10728+/vls+] /
Df(2L)pr2b, P[w+ barr+]; and
Df - Df(2L)be408. (B) Developmental expression profile of
Vls. vlsnull ovary extracts serve as a control for the
specificity of the 
-Vls antibodies. Other protein extracts were
collected from wild-type flies. The faint low molecular weight band does not
appear to originate from ovaries or embryos.