Fig. 5. Distribution of Vls-eGFP in ovaries and embryos. Transgenic flies express two copies of vls-eGFP. Anterior is towards the left, posterior towards the right. Vls-eGFP is cytoplasmic. (A) In the germarium, Vls-eGFP signal is found predominantly in the germline and at lower levels also in the somatic follicle cells, and occasionally accumulates in aggregates up to stage 1 egg chambers. Vls-eGFP is uniform in the nurse cells and the developing oocyte throughout the rest of oogenesis. (B) Stage 1-4 egg chambers, (C) stage 8 and (D,E) stage 10. Vls-eGFP signal is generally fairly weak in the oocyte and more difficult to detect against the autofluorescent yolk particles (D). However, the signal is still significantly higher than in control yw ovaries without vls-eGFP (E). (F,G) 0- to 1-hour-old embryos from vls-eGFP (F) and yw (G) mothers. (H,I) 1- to 2-hour-old embryos from vls-eGFP mothers. Vls-eGFP signal is detected in somatic and germ cell cytoplasm. pc, pole cells; sc, somatic cells.