Fig. 6. Ectopic Ser1 expression in N^ICD-IRES-GFP transfected cells. Embryos electroporated at stage HH 13 (E2) with N^ICD-IRES-GFP construct. (A) Whole-mount immunostaining 48 hours post-electroporation. Ser1 is detected in its normal broad ventromedial domain of the otocyst, with a clear limit of expression (dashed line). It is also detected ectopically in N^ICD-IRES-GFP transfected cells (arrows). (B) Transverse cryosections 5 days post-electroporation. The saccular macula shows normal Ser1 expression, while ectopic Ser1 expression is seen in a cluster of GFP-positive transfected cells (arrow). S, saccular macula.