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Fig. 1. Wild-type female gametophyte development in Arabidopsis. (A) Scheme showing the sequential developmental events leading to the wild-type female gametophyte formation in Arabidopsis. (B) Wild-type embryo sac containing one functional megaspore. (C) Wild-type embryo sac at the two-nuclear stage. Arrowheads indicate nuclei. (D) Wild-type embryo sac at the four-nuclear stage. Arrowheads indicate nuclei. (E) Cellularized wild-type embryo sac containing eight nuclei. (F) Wild-type embryo sac containing seven cells and seven nuclei. AC, archesporial cell; Ap, antipodal cells; Cc, central cell; Ccn, central cell nucleus; Ec, egg cell; FG5, eight-nuclear embryo sac; FG6, seven-cell embryo sac; FG7, four-cell embryo sac; Fm, functional megaspore; MMC, megaspore mother cell; Pn, polar nuclei; Syn, synergide; Vac, vacuole. Scale bar: 25 µm.