Fig. 5. Behavioral analysis and ERG recordings of pcdh15b morphants. (A) Optokinetic responses were measured as a function of spatial frequency of a moving square pattern. Larvae injected with 40 ng ATG Mo (filled circles) have reduced contrast sensitivity and visual acuity compared with control injected larvae (filled squares). Larvae injected with 30 ng ATG Mo (open circles) show a slightly reduced visual performance. (B) pcdh15b knock-down results in significantly reduced retinal sensitivity to light under dark- and light-adapting states. The ERG b-wave amplitudes in the dark-adapted (B) and light-adapted state (C) are plotted as a function of relative light intensity. Uninjected larvae (open squares), larvae injected with control MO (closed squares), 40 ng ATG Mo (closed circles) and 16 ng GT Mo (triangles) were analyzed. Averaged data are plotted with error bars showing mean±s.e.m. (D) RT-PCR analysis of aberrant splicing of pcdh15b transcript. The upper gel contains products amplified with primers flanking exon 4. The lower band is the predicted product without exon 4. The uppermost band presumably includes an extra intronic sequence. The lower gel shows a control reaction with elongation factor -1alpha. Lane 1, GT Mo-injected larvae, day 3; lane 2, uninjected larvae, day 3; lane 3, GT Mo-injected larvae, day 4, lane 4, uninjected larvae, day 5 (pool of 5 larvae per lane). Representative examples of ERG records from four different specimens under dark-adapted (E) and light-adapted states (F). Each trace is an average of 3-7 consecutive responses. Stimulus duration was 100 mseconds. Attenuated light intensity was presented as OD (optical density unit).