Fig. 1. Alignment of Cos2 sequences from insects and vertebrates. The nucleotide- (N4, P, N2 and N3) and microtubule-binding (M1, M2) motifs in the motor domain are different colours. The amino acid sequence corresponding to the degenerate primer pairs used to obtain a 480 bp nucleotide fragment of zebrafish cos2 are underlined. Accession numbers: AAB66813(Drosophila), XP_309818 (Anopheles), XP_133575 (mouse), XP_226596 (rat, not shown) and NP_940927 (human). Sequence of all Cos2 proteins other than that of Drosophila and zebrafish are predicted by automated computational analysis with supporting mRNA/EST evidence available for all, except Anopheles. Predictions for Anopheles and human Cos2 sequences are incomplete.