Fig. 8. Zebrafish Cos2 physically associates with Gli1. (A-C) Western blots of lysates from 293T cells transfected with GFP-Cos2 (A), Su(fu)-GFP (B) and His-Gli1 (C) constructs, showing the expected bands (marked with asterisk) of the fusion proteins (lane 2). Untransfected 293T cell lysates served as controls (lane 1). In contrast to Cos2 and Gli1, we consistently observed higher levels of Su(fu) protein expression in these transfection experiments. (D) Lanes 1-6 show lysates from untransfected 293T cells (1) or those transfected with various constructs (2-6), immunoprecipitated with anti-GFP and probed with anti-His antibodies, respectively. The expected 220 kDa band of His-Gli1 (marked with asterisk) is observed only in lanes 5 and 6.