Fig. 5. Methylation analysis at three DMRs of the Cdkn1c locus in the absence of Lsh. The methylation status was determined as outlined in Fig. 4. The arrow indicates the transcription start site. Each row corresponds to an individual sequenced strand of DNA, and each circle represents a CpG on the strand, red circles and blue circles indicate methylated and unmethylated sites, respectively. Maternal (M) and paternal (P) alleles were distinguished by a DNA polymorphism in the DMR sequences (129/Czech: 5' DMR Cdkn1c (ICG5) 34012 T/G; KvDMR1: ICG8a 101803 A/G and ICG8b 103181 G/C).