Fig. 6. Binding of Lsh to the DMR of Cdkn1c. Chromatin immunoprecipitation assays were performed in 3T3 cells that had stably integrated the Flag-Lsh expression plamids. Specific antibodies directed against the Flag-tagged form of Lsh and murine control antibodies of the same isotype were used. Aliquots of chromatin taken before immunoprecipitation were used as `Input' controls. The immunoprecipitates obtained were PCR amplified using the primers specific to the DMRs of indicated genes.