Fig. 1. Molecular lesions in dlp^MH20 and dally^MH32 and organisation of the tagged glypicans used. (A) Diagram of the genomic regions of dlp and dally. The position of the original P-elements is shown with an inverted triangle. The deletions in dlp^MH20 and dally^MH32 are indicated as small black bars (to scale). The exact break points relative to the predicted start of transcription are -42 to +467 for dlp (ATG is at 454) and -472 to +1410 for dally (ATG is at 728). The deficiencies used in complementation assays uncover the whole region with break points located far away and are represented as long black lines [Df(3L) ED4413 dally was generated for this study while Df(3L) ED4543 dlp was obtained from Drosdel]. (B) The tagged glypicans used in this study. The location and nature of the tag is shown. HA-Dally was only expressed in cultured cells while Flag-Dally and Dlp-HA were expressed in transgenic flies and cells.