Fig. 5. Cell-autonomous and non-cell-autonomous effects of Dlp overexpression. (A,B) Overexpression of Dlp in broad domains causes the loss of margin tissue. Here, overexpression was activated in the posterior compartment with engrailed-gal4 (A) or in the central region of the wing with dpp-gal4 (B). Notching is localised to where the margin overlaps with the region of overexpression (arrowheads). Details of the margin are shown on the right-hand side. (C,D) Overexpression of Dlp in scattered clones (Flp-on Dlp) leads to the formation of ectopic bristles (also to loss of margin tissue, not shown here). (C) Ectopic bristles (arrows) caused by random unmarked clones. (D) Margin area near a clone marked with the pwn mutation (outlined). An ectopic bristle (pwn⁺, i.e. outside the clone) is seen at the edge of the clone (arrow). (E,F) Overexpression of Dlp causes local accumulation of Wingless at the cell surface. Overexpression was induced in clones marked by ß-galactosidase (E). Wingless accumulation is seen in F (visualised by anti-Wingless antibody) (white arrows).