Fig. 4. MZ612+ persistent projection neurons prune processes locally during early metamorphosis. (A-H) Timecourse of pruning of (A-D) dendrites and (E-H) axons of the GAL4-MZ612-positive PPN. (I-K) Quantification of pruning scores of MZ612-positive PPN dendrites in the (I) larval AL, (J) MB calyx and (K) LH every 2 hours APF. Experimental conditions and figure labeling are as in Fig. 3. In addition to strongly labeling a single PPN that eventually innervates VA6 in adult, GAL4-MZ612 also labels other cells and processes near the larval AL, MB and LH. However, confocal tracing allows unambiguous distinction of PPN dendritic and axonal processes.